Persistent airway colonization by Candida glabrata may be an obstacle to successful weaning from a mechanical ventilator in critically ill patients. A 68-year-old man after ischemic stroke developed persistent bronchial colonization with Candida glabrata that persisted despite systemic antifungal therapy. Administration of aerosolized liposomal amphotericin B (Ambisome®) in the form of 10 mg twice a day for 10 days led to a significant improvement in respiratory mechanics. The patient was subsequently successfully weaned from the ventilator. Microbiological cultures confirmed the disappearance of colonization after treatment. This approach has been shown to be effective and well tolerated even in the non-immunosuppressed patient.