The study compared the antibiotic resistance and molecular characteristics of Escherichia coli isolated from patients with asymptomatic bacteriuria (ASB), urinary tract infection (UTI) and uropathic bloodstream infection (BSI). Fifty cases were included in each group: BSI with concurrent bacteremia (BSI-B), UTI without bacteremia (UTI-U), and ASB (ASB-U). UTI-U isolates had significantly higher resistance to the antibiotics aztreonam (28% vs. 8%), ceftazidime (20% vs. 4%), cefepime (16% vs. 2%), and gentamicin (30% vs. 0%) compared to the BSI-B group. Phylogenetic group B2 and sequence type ST131 dominated the BSI-B and UTI-U groups, while ST1193 predominated in the ASB-U group. Gene factors of virulence, such as exotoxins (hlyABCD, cnf1) and adhesion genes (papBCDEFGHJK), were more frequent in BSI-B and UTI-U groups than in ASB-U. In addition, the BSI-B group showed a higher presence of iroBCDEN nutritional/metabolic factor transfer genes. These differences in resistance and molecular characteristics may help in targeted treatment of patients with different forms of E. coli infections[4].