A cross-sectional study among 385 healthcare professionals in Saudi Arabia assessed knowledge, counseling practices and educational gaps about drug-food interactions (DFIs). A total of 231 respondents (60.0%) had limited knowledge of DFI mechanisms. More than half (51.4%) had not received any formal instructions about DFI, and 146 participants (37.9%) relied on informal sources of information. Only 164 professionals (42.6%) routinely advise patients about DFI involving traditional foods or herbal products. Only 73 participants (19.0%) ever reported an adverse event related to DFI. The findings show deficiencies in curriculum, professional training and cultural alignment of practices.