Construction and validation of nomogram prediction model for recurrent spontaneous abortion based on the expression of MALAT1, miR-515-5p, and MCL1 mRNA

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Source: Frontiers Medicine

Original: https://www.frontiersin.org/articles/10.3389/fmed.2025.1558818...

Published: 2025-12-17T00:00:00Z

The researchers constructed and validated a nomogram prediction model for recurrent spontaneous abortion (RSA) based on MALAT1, miR-515-5p and MCL1 mRNA expression in 135 patients in the training set and 58 in the validation set. Independent risk factors for RSA were advanced maternal age, more than 3 abortions, low progesterone level at the beginning of pregnancy, high MALAT1 expression, low miR-515-5p expression, and low MCL1 mRNA expression (p < 0.05). The nomogram model had a C-index of 0.915 in the training set and 0.869 in the validation set. The calibration curve showed good agreement between predicted and actual values ​​with mean absolute errors of 0.012 and 0.034; The Hosmer-Lemeshow test had p values ​​of 0.362 and 0.779. The ROC curve achieved an AUC of 0.916 (95% CI 0.858–0.974) in the training set and 0.867 (95% CI 0.742–0.992) in the validation set. Decision curve analysis confirmed the clinical value of the model. MCL1 mRNA is useful for early-stage RSA prediction and clinical decisions.