Multistep genomics on single cells and live cultures in subnanoliter capsules

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Source: Science Magazine

Original: https://www.science.org/doi/abs/10.1126/science.ady7209?af=R...

Published: 2025-12-18T07:00:42Z

Scientists have developed capsules with amphiphilic gel envelopes (CAGEs) that selectively retain cells and large analytes, but are freely accessible to media, enzymes, and reagents.[1][2][4] These capsules enable high-throughput multistep analyzes that combine live cell culture with genome-wide genomic readouts.[1][2][4] Single cell sequencing methods reveal natural and induced variability between cells.[1][2][4] They developed methods for barcoding DNA libraries in CAGEs.[1][2][4] They applied these methods to measure the persistence of gene expression programs in cells by capturing the transcriptomes of tens of thousands of expanding clones in CAGEs.[1][2][4] The compatibility of CAGEs with various enzyme reactions allows the expansion of the repertoire of high-throughput measurements at the level of individual cells and their application to living cells.[1][2][4]