Researchers have developed semipermeable capsule (SPC) technology for high-throughput single-cell omics analyzes of nucleic acids, including genome and mRNA sequencing at the single-cell level.[1][2] The capsules have a liquid core surrounded by a thin semipermeable membrane that allows small molecules such as enzymes, reagents, and nutrients to pass through, but retains larger molecules such as DNA and RNA.[3][4] The size of the capsules varies from 35 to 260 μm and the thickness of the shell can be adjusted, typically 70 ± 2 μm with a thickness of 2.2 μm.[4] SPCs are biocompatible, allowing long-term cultivation of single-cell cultures and clonal expansion for weeks, overcoming the limits of liquid microfluidic platforms.[1][2] The CapSeq procedure for high-throughput RNA sequencing with combinatorial barcoding and RNA marker-based capsule sorting using fluorescence-activated cell separation are introduced.[3] The technology enables sequential reactions, reagent exchanges, and sorting without loss of single-cell identity, with robust enrichment of positive cells, such as leukemia blasts, in patient samples.[3][4] SPCs provide flexibility and scalability over existing droplet or microtiter plate methods.[1][3]