The study examined the anatomy of the lateral part of the face, particularly the superficial musculoaponeurotic system and the course of the facial nerve, to evaluate the safety of threading in facelift. They used eight fresh cadavers where threads were inserted through a single entry point in the temporal region. They inserted three sutures on each side: one ending 1 cm lateral to the corner of the mouth and the other 1 cm lateral to the first endpoint. Safety was assessed using micro-CT with contrast agent phosphotungstic acid and ultrasonography. Threads were successfully introduced into the layer superficial to the superficial musculoaponeurotic system without damage to blood vessels, nerves or muscles. This layer is anatomically advantageous for lifting the lateral tissues of the face and minimizes the risk of complications.