A cross-sectional study conducted in August and September 2022 in four hospitals in Shandong Province investigated the artificial intelligence readiness of 329 newly qualified nurses with 1–3 years of experience. Readiness was moderate (mean 9.85, standard deviation 1.97). Multiple linear regression identified three significant factors: perceived ease of use (β = 0.211, p = 0.006), previous AI training (β = 0.23, p < 0.001), and awareness of AI in nursing practice (β = 0.201, p = 0.018). Perceived barriers did not significantly affect readiness, but in practice, lack of knowledge about AI and limited computer skills were common. The study used a technology acceptance model augmented with perceived barriers and analyzed the data using SPSS 27.0. AI training, better systems usability and resource support are essential to improve readiness.