Endometriosis is a chronic gynecological disease characterized by the growth of endometrial-like tissue outside the uterus, which causes pain and infertility and impairs quality of life. The diagnosis of endometriosis is delayed due to the reliance on invasive laparoscopy and the lack of sensitive and specific non-invasive biomarkers. Current molecular and imaging methods have improved detection but still have limitations. The article presents a mechanobiological perspective that examines how the biophysical properties of cells—such as stiffness, deformability, and contractility—could be used as functional biomarkers of endometriosis. Research focuses on lesion subtypes, menstrual cycle dynamics, and biological processes including decidualization, epithelial-mesenchymal transition, and stromal remodeling. Menstrual discharge is identified as a promising non-invasive cell-rich sample suitable for mechanical profiling. Tracking endometriosis with a mechanical lens could accelerate diagnostic innovations and reveal new mechanisms driving the development and progression of the disease.