Atopic dermatitis is a chronic inflammatory skin disease driven by memory T cells homing to the skin. The study analyzed subpopulations of memory T cells in the peripheral blood of adult AD patients according to disease severity using flow cytometry. CD4+ and CD8+ memory cells with skin homing markers CLA, CCR4 and CCR10 were evaluated. The distribution of total memory CD4+ and CD8+ T cells was preserved. AD patients had an expansion of CD4+ central memory T cells expressing CLA, CCR4 and CCR10. The circulating population of CD8+ ex-Trm cells co-expressing CLA, CCR4 and CCR10 increased in moderate to severe disease and correlated with clinical severity. There was no similar expansion in CD4+ ex-Trm cells. These cells may serve as potential biomarkers of disease severity and spread.