This systematic review and meta-analysis investigated the prognostic significance of vimentin expression in tumor cells in pancreatic ductal adenocarcinoma (PDAC). Of 443 articles, 9 studies were included after screening the PubMed, Web of Science, and Scopus databases until July 8, 2025. Vimentin expression is associated with reduced overall survival (OS): pooled logHR = 1.39, 95% CI (0.11, 2.68), p = 0.036, with high heterogeneity (p < 0.001). It was also significantly associated with higher lymph node stage (N-stage): pooled logOR = 0.58, 95% CI (0.08, 1.08), p = 0.022, with zero heterogeneity (I² = 0%, p = 0.418). There was no association with T-stage (pooled logOR = -0.10, 95% CI: (-0.87, 0.66), p = 0.791) or M-stage (pooled OR = 0.08, 95% CI: (-0.95, 1.12), p > 0.05). For OS, there was asymmetry of the funnel plot, indicating possible publication bias. Registration: PROSPERO CRD420251127404.