Refractory non-erosive reflux disease (rNERD) is a type of esophageal reflux that does not improve with proton pump inhibitors and is caused by increased sensitivity of the esophagus. The study examined the effect of radiofrequency ablation (RFA), an endoscopic procedure, in 20 patients with rNERD. After the procedure, the expression of PAR2 and TRPV1 receptors, which are responsible for the sensitivity of the esophagus, was significantly reduced - PAR2 decreased from 81.8% to 35.5% and TRPV1 from 1.6 to 0.9. At the same time, inflammatory infiltration in the esophagus tissue was reduced. Patients reported improvement in symptoms, with Reflux Symptom Index scores falling from 14.6 to 5.0 and GERD-Q scores from 14.0 to 7.6. The results suggest that radiofrequency ablation alleviates the symptoms of refractory non-erosive reflux disease in two ways: anatomical correction and reduction of esophageal hypersensitivity through modulation of sensitive receptors.