A 26-year-old woman had a 1-month history of recurrent right lower abdominal pain. She had previously been misdiagnosed with appendicitis and Crohn's disease. Intestinal tuberculosis complicated by the sinus tract of the ascending colon was confirmed by a comprehensive diagnostic work-up, including enterography by computed tomography of the small intestine, the test of purified protein derivatives, colonoscopy, and pathological evaluation. Quadruple antituberculosis treatment was started. The fistula achieved complete closure 6 months after treatment. This condition is characterized by nonspecific symptoms and requires multidisciplinary cooperation with a multidimensional differential diagnostic approach. The case points to the importance of multidisciplinary diagnosis and treatment in intestinal tuberculosis. Provides reference material for clinical diagnosis of rare complications.