Autophagolysosomal exocytosis is the major pathway by which Src family kinases reach the cell surface in breast cancer.[1] The study shows that Src kinase non-canonically inverts to the cell surface in cancer cells both in vitro and in vivo.[4] This process involves autophagolysosomes, where Src associates with the autophagosome marker LC3, as confirmed by microscopic images of HCC1569 cells.[4] Experiments include inhibitors such as ATG7-IN-2 to block autophagosomal formation and siRNA targeting the SRC gene, analyzed by fluorescent immunoblots with tubulin as a control.[4] The results demonstrate a role for autophagolysosomal exocytosis in the translocation of Src kinase to the surface in cancer cells.[1][4]