T Cell Receptor repertoire analysis reveals antigenic convergence and immunotherapeutic opportunities in Prostate Cancer

The study analyzed T-cell receptors (TCR) from tumor tissues and peripheral blood of treatment-naive prostate cancer patients. Results showed that tumor TCR repertoires had lower diversity and higher clonality compared to peripheral blood, indicating local antigen-driven expansion. High-grade tumors demonstrated greater clonotype sharing between patients and motif convergence, suggesting recognition of common tumor-associated antigens. Analysis identified three potential targets: PSGR (prostate-specific G-protein coupled receptor), PSMA (prostate-specific membrane antigen), and PSA (prostate-specific antigen). Functional tests confirmed that peptides from PSGR and PSMA induced interferon-gamma production and antigen-specific T-cell proliferation in vitro. These findings indicate that PSGR and PSMA are immunogenic and potentially actionable targets for immunotherapy in prostate cancer.